Question: URGENTTT! As One Of The Members Of The R&D Team Which Is Working On The Development Of A Biosensor For Ketoacidosis, You Would Like To Propose A New Biosensor That Is Based On The Electrochemical Mode. This Biosensor Is Able To Measure The Level Of Ketone In Blood. The High Levels Of Ketone In Blood Serum Can Cause Life-threatening Ketoacidosis. The …
As one of the members of the R&D team which is working onthe development of a biosensor for ketoacidosis, you would like topropose a new biosensor that is based on the electrochemical mode.This biosensor is able to measure the level of ketone in blood. Thehigh levels of ketone in blood serum can cause life-threateningketoacidosis. The new biosensor comprises 3-hydroxybutyratedehydrogenase (3-HBDH) and graphene. 3-HBDH catalyses the followingreaction as shown below.
3-hydroxybutyrate (3-HB) + NAD+ → acetoacetate + NADH
(a) You are aware that a biosensor should have a chemicalrecognition element and a transducer. Describe the electrochemicalbiosensor that you plan to propose to the R&D team.
(b) Briefly explain the immobilisation of the chemicalrecognition element for the biosensor mentioned in question Q1